Ultra Performance Liquid Chromatography (UPLC Analysis)

Microtrace offers sample analysis by ultra (high) performance liquid chromatography (UPLC/UHPLC). This laboratory technique allows us to separate and analyze components of a mixture based on their hydrophobicity. The process is similar to traditional high performance liquid chromatography (HPLC). However, the higher pressure of UPLC permits us to analyze even smaller samples and obtain higher resolution separation.

In the UPLC instrument, separated compounds pass through a photo diode array (PDA). This detects and measures their absorption profile. Our experts use this absorbance spectrum and retention time data to identify and quantify components of the separated compound.

Our scientists use liquid chromatography in conjunction with other techniques for the testing of unknown samples, including trace contaminants. After using UPLC to isolate, purify, and/or accumulate (or concentrate) the components of a specimen, we can then analyze the sample by other methods in our lab. For instance, the following techniques often supplement UPLC analysis: Raman spectroscopy, mass spectroscopy, infrared spectroscopy and thermal analysis.  Microtrace’s UPLC also permits reversed-phase LC and the analysis of non-volatile and semi-volatile samples that are soluble in non-polar solvents (e.g., water or alcohol).

Liquid chromatography can help solve problems in a wide-range of fields from forensic investigations to manufacturing. This instrument can assist with the analysis of chemicals and drugs (pharmaceutical and illicit), ingredients, explosive residues, toxicological samples, environmental samples, contaminants, and unknown materials. Contact our lab to learn how UPLC analysis can assist with your problems.

Whether it’s a chemical screening, compliance evaluation, contamination analysis, failure analysis, or forensic investigation, Microtrace is here to help. Our decades of experience and state-of-the-art laboratory allow us to address the specific needs of our clients, with both customized and standardized methods.

UPLC Instrumentation:

Waters Acquity H-Class Plus- Quaternary pump- eLambda PDA (190-800 nm, 1.2 nm res.) – fraction manager

 

Related Standardized Methods: ASTM E2036, E2037

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